Hi everyone, Now that I have reinstalled mira and reloaded the data in the bin directory of mira, now I can run mira, with your guidance. still mira = 0 bytes, and log file shows AS_readpool: 0 reads. AS_contigs: 0 contigs. AS_bbcontigs: 0 contigs. Mem used for reads: 60 (60 B) and Warning: "cotton_backbone_in.gbf" : File not found. ->Thrown: GBF::load(const string & gbfin) ->Caught: main why this conclusion, when I already downloaded the respective gb file of the cotton data, renamed as .gbf and stored it in the gbf directory as directed in the manual. Although ls -l shows me drwxr-xr-x 2 sharmistha sharmistha 4096 2009-10-02 13:15 Cotton_backbone_in.gbf drwxr-xr-x 2 sharmistha sharmistha 4096 2009-10-02 13:15 cotton_d_info drwxr-xr-x 2 sharmistha sharmistha 4096 2009-10-02 13:15 cotton_d_log drwxr-xr-x 2 sharmistha sharmistha 4096 2009-10-02 13:15 cotton_d_results -rw-r--r-- 1 sharmistha sharmistha 243851317 2009-09-16 11:03 cotton.fasta -rw-r--r-- 1 sharmistha sharmistha 386595241 2009-09-16 10:56 cotton.fastq -rw-r--r-- 1 sharmistha sharmistha 132828265 2009-09-19 12:58 cotton_straindata_in.txt -rw-r--r-- 1 sharmistha sharmistha 22289 2009-10-02 13:15 log_assembly.txt -rw-r--r-- 1 sharmistha sharmistha 0 2009-09-24 11:34 mira but, the respective directory with 4096 bytes, actually contains nothing inside it leaving the gbf directory . Mira actually is not running properly is my conclusion, I also feel it is not able to read through my ref file, so it cannot draw the respective contigs. If so, what is the reason for it? By chance if I am wrong, If the flaw lies elsewhere, please can you guide where the error actually lies? Waiting for some interesting replies Thanks and Regards, Sharmistha On Thu, Oct 1, 2009 at 10:46 AM, Sharmista Saha < sharmistasaha@xxxxxxxxxxxxxx> wrote: > Thanks a lot to both Peter and Bjorn. > > Regards, > Sharmistha > > > On Wed, Sep 30, 2009 at 10:00 PM, Bjoern Usadel > <usadel@xxxxxxxxxxxxxxxxx>wrote: > >> Dear Sharmista, >> >> The best bet would probably to contact the relevant PI for the Gossypium >> projects: It seems finished but not yet released. >> >> http://www.jgi.doe.gov/sequencing/statusreporter/psr.php?projectid=16065 >> >> >> Cheers, >> Björn >> >> Peter wrote: >> >>> On Wed, Sep 30, 2009 at 4:11 PM, Sharmista Saha >>> <sharmistasaha@xxxxxxxxxxxxxx> wrote: >>> >>> >>>> Hi, >>>> >>>> @Peter, thank you, for your suggestion, even myself tried with that >>>> earlier, >>>> but I do not find Gossypium, where can i get that gb file of the same? >>>> >>>> >>> >>> You just asked for Arabidopsis thaliana - not cotton ;) >>> >>> I tried searching the genomes at the NCBI with Entrez for Gossypium, >>> but just found two chloroplasts: >>> >>> http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&term=Gossypium[orgn]<http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&term=Gossypium%5Borgn%5D> >>> >>> I am not a plant geneticist, so I have no idea if the whole genome has >>> been sequenced, and if it has, where else to look for it. >>> >>> Peter >>> >>> >>> >> >> -- >> -------------------------------------------------- >> Björn Usadel, PhD >> Max Planck Institute of Molecular Plant Physiology >> AG Integrative Carbon Biology >> Am Muehlenberg 1 >> 14476 Potsdam-Golm >> Tel.: +49 331 5678153 >> email usadel@xxxxxxxxxxxxxxxxx >> http://tinyurl.com/IntegrativeCarbonBiology >> -------------------------------------------------- >> >> >> >> -- >> You have received this mail because you are subscribed to the mira_talk >> mailing list. For information on how to subscribe or unsubscribe, please >> visit http://www.chevreux.org/mira_mailinglists.html >> > >