Thanks for the quick response. ans: 1. I do have all the respective files in the folder I am running mira. 2. They do all have the correct names. Eg: mydata.fasta; mydata.fastq; mydata_backbone_in.gbf; mydata_straindata_in.txt, my seq.gbf 3. I ran the respective command you provided, unfortunately, error says: bash: mira: command not found. Now can it be easier for you to guide me to the root of my errors? Please I am waiting. Thanks and Regards, Sharmistha On Wed, Sep 30, 2009 at 12:40 PM, Davide Sassera (davide.sassera) < davide.sassera@xxxxxxxx> wrote: > Dear Sharmista, > > 1: do you have all the necessary files in the folder you are running mira? > > 2: do they have all the correct names? please provide a list of the files > > 3: I think The command should be: > > mira -project=my_project -job=mapping,genome,accurate,solexa -AS:nop=1 > -SB:lsd=yes:bsn=myproject_ref:bft=gbf:bbq=30 > > Ciao > D. > > > ----- Messaggio Originale ----- > Da: Sharmista Saha <sharmistasaha@xxxxxxxxxxxxxx> > Data: Mercoledi', Settembre 30, 2009 8:58 am > Oggetto: [mira_talk] Re: Assembly > A: mira_talk@xxxxxxxxxxxxx > > > > The command I typed was as follows: > > sharmistha@PLEX- > > 1:~/Sharmistha/Bioinfo/Project_data/my_project/mira/Dataform>mira > > --project=my_project --job=mapping,genome,accurate,solexa* > > *-AS:nop=1 > > > > * -SB:lsd=yes:bsn=myproject_ref:bft=gbf:bbq=30 > > >&log_assembly.txt } > > > > > > > > *This was my comand line.......... > > > > now can you suggest me, where am I wrong? > > > > Thanks and Regards, > > > > Sharmistha > > > > > > > > On Wed, Sep 30, 2009 at 12:14 PM, Sven Klages > > <sir.svencelot@xxxxxxxxxxxxxx>wrote: > > > > > You still didn't provide the command you typed in. So it's > > difficult to > > > tell what went wrong. > > > > > > cheers, > > > Sven > > > > > > 2009/9/30 Sharmista Saha <sharmistasaha@xxxxxxxxxxxxxx> > > > > > > Can anyone solve my required issue? I need it very much and am > > very curious > > >> to see how my denovo assembly happens via mira,. > > Please, if anyone can > > >> direct me my error in execution of mira, I shall be highly > > grateful. I am > > >> still positive about the tool to work in my sequence too, > > when it can > > >> resolve so many queer issues. > > >> > > >> Only my sequence is a solexa sequence. waiting > > hopefully for some > > >> positive response on the same ground. > > >> > > >> Thanks and Regards, > > >> Sharmistha > > >> > > >> > > >> On Fri, Sep 25, 2009 at 2:06 AM, Bastien Chevreux > > <bach@xxxxxxxxxxxx>wrote:>> > > >>> On Donnerstag 24 September 2009 Sharmista Saha wrote: > > >>> > [...] > > >>> > This is what I got. Hope this answers your query. > > >>> > > >>> Not entirely, you forgot the command line you typed in. > > Fortunately, the > > >>> directory listing you provided gives a hint to what happened. > > >>> > > >>> Do NOT type the command line prompt that is shown in the > > examples, it's > > >>> NOT > > >>> part of the mira command! > > >>> > > >>> Hope that helps. > > >>> > > >>> Regards, > > >>> Bastien > > >>> > > >>> > > >>> -- > > >>> You have received this mail because you are subscribed to > > the mira_talk > > >>> mailing list. For information on how to subscribe or > > unsubscribe, please > > >>> visit http://www.chevreux.org/mira_mailinglists.html > > >>> > > >> > > >> > > > > > >