-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Hi, I am using sff_extract on a 454 .sff file, and I have realized some adapters (probably MIDs) are still present: a left adapter (acactactcgt) is present in 99% of reads and a right adapter (acgagtggtgt) is present in 14% of reads. I manage to clip the left end with --min_left_clip=16 but not the right end. Does anyone know how to clip the right end of reads? Better even, how to right clip a specific sequence? Thanks in advance, Best, Leonor. -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.10 (GNU/Linux) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org/ iQEcBAEBAgAGBQJPFYXfAAoJEKquFGwgRb3zV3gH/3Gu57k15F25ZoFWe6a0LSR2 Qo4bXPOPyyRLbebq/oqVa2T8A7aBJhVNUC9gupaksflt4FdewNqaltgmxVKzzXgd oGO1AzDiDxTZDWSFsEX51hQ2qiGBp+zOZDkTMLkfWZ5PXIFN0wPV7QUt14W/nOd8 c0agfqsVA+OZQ72mj6+vb8s9d+IYPN9c3bVy2krl5bUIrIuD+19nXSabGGEDDvCM n4tr2ZieS0tXA8YyiNZop4Zq0C3sJygzBz/JITYYKXwYKwjC1X/jQ0Mfl4GXIVct THZe+2K/AeQ8Jz4TpwLyFsq+PfIT/zazO/RKFmTfjqbm/ovARSzRhnZMvDL17xQ= =Ja1v -----END PGP SIGNATURE----- -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html