[mira_talk] 454 trimming with sff_extract

  • From: Leonor Palmeira <mlpalmeira@xxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Tue, 17 Jan 2012 15:29:51 +0100

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Hi,

I am using sff_extract on a 454 .sff file, and I have realized some
adapters (probably MIDs) are still present: a left adapter (acactactcgt)
is present in 99% of reads and a right adapter (acgagtggtgt) is present
in 14% of reads.

I manage to clip the left end with --min_left_clip=16 but not the right end.

Does anyone know how to clip the right end of reads? Better even, how to
right clip a specific sequence?

Thanks in advance,
Best,
Leonor.
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