[ascct] Launch of 2 OECD Test Guidelines on human hazard endpoint skin sensitisation

  • From: Kristie Sullivan <KSullivan@xxxxxxxx>
  • To: "ascct@xxxxxxxxxxxxx" <ascct@xxxxxxxxxxxxx>
  • Date: Fri, 6 Feb 2015 09:05:34 -0500

6/2/2014 - The OECD has just published two new Test Guidelines on human health 
hazard endpoint skin sensitisation. Skin sensitisation refers to an allergic 
response following skin contact with the tested chemical, as defined by the 
United Nations Globally Harmonized System of Classification and Labelling of 
Chemicals (UN GHS). Test Guideline 442C concerns an In Chemico procedure for 
the Direct Peptide Reactivity Assay and addresses the molecular initiating 
event leading to the skin sensitisation. Test Guideline 442D looks at an in 
vitro procedure, namely the ARE-Nrf2 luciferase test method, and looks to 
address the second key event on the adverse outcome pathway leading to skin 
sensitisation. Both of these Test Guidelines set out the procedures for 
supporting the discrimination between skin sensitisers and non-sensitisers in 
accordance with the UN GHS. They are the first two in a series of in vitro 
methods that will gradually replace in vivo animal Test Guidelines on skin 
sensitisation; more of these alternative methods are detailed in the work plan 
of the Test Guidelines 

·         Test No. 442C: In Chemico Skin Sensitisation (Direct Peptide 
Reactivity Assay 

·        Test No. 442D: In Vitro Skin Sensitisation (ARE-Nrf2 Luciferase Test 

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  • » [ascct] Launch of 2 OECD Test Guidelines on human hazard endpoint skin sensitisation - Kristie Sullivan