> ..Hi Jim, > > I also use these buffers regularly (actually right now), and I always > understood that the EDTA was essential for the conductivity(?) during > electrophorsis, but how this exactly works I cannot remember..., but > omitting EDTA gives terrible running behaviour of DNA molecules..ok > enough off-topic..;-).. > I don't do electrophoresis here but the properties of the pre-packaged buffer as sold by Sigma was too good to pass up for photography. It comes in 1L pouches and makes 1000ml buffer that is about 500mM (0.5M). This can be diluted 1:5 or 1:10 for use in developers, unless you want a more alkaline reagent. Can't think why Ca and/or Mg sequestration might improve DNA mobility in gel... this might have implications for photography as the gelatin layer is a gel also and silver halide/sliver granules may have some degree of mobility (undesirable, as it might lead to graininess) but no electric field is applied to the film (not on purpose that is). JB ============================================================================================================= To unsubscribe from this list, go to www.freelists.org and logon to your account (the same e-mail address and password you set-up when you subscribed,) and unsubscribe from there.