From: "John Black" <jblack@xxxxxxxxx> Subject: [pure-silver] Re: Pyro in a Jobo Processor Date: Fri, 18 Mar 2005 12:43:48 -0500 > The presence or absence of the EDTA seems to have no noticable > effect on the rate of oxidation of sulfite in my hands. My buffer > happens to contain EDTA but I think it would perform as well without > it (see below). That's not a good test. Also, if you still use tris buffer, tris buffer has some affinity to metal ions and has similar action to potentate redox catalysis of iron. Phosphate is another bad choice. For pH of 7.3-8.3 range I find triethanolamine to be a very good buffering agent although it does not abolish iron catalysis in this pH range. -- Ryuji Suzuki "Keep a good head and always carry a light camera." ============================================================================================================= To unsubscribe from this list, go to www.freelists.org and logon to your account (the same e-mail address and password you set-up when you subscribed,) and unsubscribe from there.