[proteomics] Re: Instaview Nitrocellulose

  • From: "Ipek Bosgelmez" <ipek4477@xxxxxxxxx>
  • To: proteomics@xxxxxxxxxxxxx
  • Date: Mon, 25 Dec 2006 21:18:55 +0200

Following links may be of interest:

#1. https://www.invitrogen.com

#2. http://www.merckbiosciences.co.uk/

   BLOT-Fast Stain™ Reagent
  *Cat. No. WB56  *  Western Blotting Detection Systems &
for Blocking, Staining, and

 *A rapid, convenient method for visualizing protein transfer to
nitrocellulose and PVDF membranes.* Formulation: 125 ml Reagent A (Fixer)
and 125 ml Reagent B (Developer) Kit Contents: Reagent A (fixer) and Reagent
B (developer)  Comments: Stains as little as 0.5 ng of protein per band.
Background remains unstained, leading to exceptional band visibility. Stain
can be easily removed with warm water, and Western blotting can proceed with
no changes in protocol. Kit includes fixer reagent and developer sufficient
to stain 25 12 X 12 cm2 blots.

Ipek Bosgelmez

On 12/25/06, Mavi Gozler <mavigozler@xxxxxxxxx> wrote:

Perhaps all if not most of you are familiar with reversible staining of
proteins electroblotted onto membranes (PVDF, nitrocellulose) to be
immunochemically illuminated (westerns).  Ponceau S has been around a long
time and still seems reliable.

BDH Laboratory Supplies (Poole UK) appears to offer something called
"Instaview Nitrocellulose."  The kit includes a concentrate of stain
(Coomassie, I think), "enhancer" (which actually improves contrast by
removing noise [background staining]), and a destain.

I could not find a home page for this manufacturer.  Perhaps they sell
through vendors (B2B).

I am also curious about the way scientists are illuminating their westerns
typically.  Do people use enzyme-conjugated secondary antibodies to produce
visible colored precipitates or do they prefer chemiluminescent (and if so,
how are you imaging them:  film or digicam?)


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