[proteomics] Instaview Nitrocellulose
- From: Mavi Gozler <mavigozler@xxxxxxxxx>
- To: proteomics@xxxxxxxxxxxxx
- Date: Mon, 25 Dec 2006 07:03:02 -0800 (PST)
Perhaps all if not most of you are familiar with reversible staining of
proteins electroblotted onto membranes (PVDF, nitrocellulose) to be
immunochemically illuminated (westerns). Ponceau S has been around a long time
and still seems reliable.
BDH Laboratory Supplies (Poole UK) appears to offer something called "Instaview
Nitrocellulose." The kit includes a concentrate of stain (Coomassie, I think),
"enhancer" (which actually improves contrast by removing noise [background
staining]), and a destain.
I could not find a home page for this manufacturer. Perhaps they sell through
vendors (B2B).
I am also curious about the way scientists are illuminating their westerns
typically. Do people use enzyme-conjugated secondary antibodies to produce
visible colored precipitates or do they prefer chemiluminescent (and if so, how
are you imaging them: film or digicam?)
SMH
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