[OGD] Responses to Super-High Atmospheric CO2 Enrichment

"Norikane, A., Takamura, T., Morokuma, M. and Tanaka, M.
2010            In vitro growth and single-leaf photosynthetic response of 
Cymbidium 
plantlets to super-elevated CO2 under cold cathode fluorescent lamps. Plant 
Cell Reports 29: 273-282.
...
The authors write that "the genus Cymbidium comprises about 50 species 
distributed in tropical and subtropical Asia and Oceania," and that "almost 
all cultivated cymbidiums are hybrids, thin-leaved and with a C3 mode of 
photosynthesis."
In addition, they say that "Cymbidium was the first orchid genus to be 
propagated using shoot-tip culture."
...
Cymbidium (Music Hour 'Maria') shoots with two to three leaves that had 
been obtained from a mass of protocorm-like bodies derived from shoot-tip 
culture were further cultured in vitro on modified Vacin and Went medium in 
air augmented with either 0, 3000 or 10,000 ppm CO2 under photosynthetic 
photon flux densities of either 45 or 75 µmol m-1 s-1 that were provided by 
cold cathode fluorescent lamps for a period of 90 days, after which they 
were transferred to ex vitro culture for an additional 30 days.
...
Relative to plants grown in vitro in ambient air, the percentage increases 
in shoot and root dry weight due to enriching the air in which the plants 
grew by 3000 ppm CO2 were, respectively,
216% and 1956% under the low light regime and 249%
and
1591% under the high light regime,
while corresponding increases for the plants grown in air enriched with an 
extra 10,000 ppm CO2 were
244% and 2578% under the low light regime
and
310% and 1879% under the high light regime.

Similarly, in the ex vitro experiment, percentage increases in shoot and 
root dry weight due to enriching the air in which the plants grew by 3000 
ppm CO2 were, respectively,
223% and 436% under the low light regime
and
279% and 469% under the high light regime,
while corresponding increases for the plants grown in air enriched with an 
extra 10,000 ppm CO2 were
271% and 537% under the low light regime
and 332% and 631% under the high light regime.
...
The four Japanese researchers conclude that "super-elevated CO2 enrichment 
of in vitro-cultured Cymbidium could positively affect the efficiency and 
quality of commercial production of clonal orchid plantlets."   "

URL : http://www.co2science.org/articles/V13/N19/B3.php

****************
Regards,

VB

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