Michael, Great response... One of the common misconception of fluorescein /vegetable dye may be a result of the following quote cited in our sometimes reliable Wikipedia Uses in river systems One of its more recognizable uses was in the Chicago <http://en.wikipedia.org/wiki/Chicago_River> River, where fluorescein was the first substance used to dye the river green on St. Patrick's Day <http://en.wikipedia.org/wiki/St._Patrick%27s_Day> in 1962. In 1966 environmentalists forced a change to a vegetable based dye to protect the thousands of goldfish that populate the river Stuart Weedn Apollo Ophthalmics