I am having issue running ESTSNP search. Here is my code: miraSearchESTSNPs --project=skt --job==denovo,normal,454 454_SETTINGS,esps1 >log_assembly_esps1.txt In my working director I have a FASTA file containing all sequences from both strains, uniquely labelled. I then have the same for the QUAL file. My straindata_in.txt file contains a list of all the sequence names, and strain name. If I put in the above code, nothing happens; jackie@ubuntu:~/Desktop/Mira$ miraSearchESTSNPs --project=skt --job==denovo,normal,454 454_SETTINGS,esps1 >log_assembly_esps1.txt jackie@ubuntu:~/Desktop/Mira$ If I then remove the creat log commant I get the following error: Parsing parameters: --project=skt --job==denovo,normal,454 454_SETTINGS,esps1 Seen no assembly quality in job definition, assuming 'normal'. Seen no assembly type in job definition, assuming 'genome'. Seen no assembly method in job definition, assuming 'denovo'. ,,, ========================= Parameter parsing error(s) ========================== * Parameter section: '(none)' * unrecognised string or unexpected character: denovo * Parameter section: '(none)' * unrecognised string or unexpected character: normal * (may be due to previous errors) * Parameter section: '(none)' * unrecognised string or unexpected character: 454 * (may be due to previous errors) * Parameter section: '(none)' * unrecognised string or unexpected character: esps1 * (may be due to previous errors) ============================================================================ === Fatal error (may be due to problems of the input data or parameters): "Error while parsing parameters, sorry." ->Thrown: void MIRAParameters::parse(istream & is, vector<MIRAParameters> & Pv, MIRAParameters * singlemp) ->Caught: main Aborting process, probably due to error in the input data or parametrisation. Please check the output log for more information. Any help would be much appreciated! Jack