[mira_talk] problem with miraSearchESTSNPs

  • From: Bastian Greshake <bgreshake@xxxxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Fri, 11 Jun 2010 10:27:11 +0200

Hi,
i'm having some trouble while trying to run the snp-detection step2. 

I'll start the first step using: 

miraSearchESTSNPs 
                                        --fastq 
                                        --project=snpassembly 
                                        --job=denovo,normal,sanger,esps1 
                                        --noclipping=sanger


This one works fine and it gives me corresponding output-files for step1. Then 
i'm trying to run step2 to using: 

 miraSearchESTSNPs 
                                        --job=denovo,normal,sanger,esps2

This step stops stating: 

> Fatal Error (may be due to problems of the input data):
> "You did not specify any input sequences to be loaded."
>
> ->Thrown: void Assembly::loadSequenceData_new()
> 
> ->Caught: main

I can't see why this happens as it looks like i use the 2nd step like the 
manual says. What could be the problem in here?

Cheers,
Bastian

 
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