Hi, i'm having some trouble while trying to run the snp-detection step2. I'll start the first step using: miraSearchESTSNPs --fastq --project=snpassembly --job=denovo,normal,sanger,esps1 --noclipping=sanger This one works fine and it gives me corresponding output-files for step1. Then i'm trying to run step2 to using: miraSearchESTSNPs --job=denovo,normal,sanger,esps2 This step stops stating: > Fatal Error (may be due to problems of the input data): > "You did not specify any input sequences to be loaded." > > ->Thrown: void Assembly::loadSequenceData_new() > > ->Caught: main I can't see why this happens as it looks like i use the 2nd step like the manual says. What could be the problem in here? Cheers, Bastian -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html