On 6 October 2011 00:12, Bastien Chevreux <bach@xxxxxxxxxxxx> wrote: > Damn. Never tested Illumina 1.0 format in FSTQ, too old. My bad. > > Your only option at the moment: convert the FASTQ to standard FASTQ. I think > one can use the FASTX toolkit for that, but I forgot how. > Ah ok, why does mira guess an offset of 59, which seems to be the problem? I know there are 3 different Solexa formats, but either they use ASCII offset of 33 or 64? best, Peter -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html