On Thursday 30 April 2009 Sucheta Tripathy wrote: > As I reported last time, the assembly output was not OK, with very shorter > fragments and the total assembled data much less than expected. Is there > any other parameter setting I can try to get a better result? Ouch, sorry to have forgotten you. I think you've been hit by a bug in 2.9.44x2: - bugfix: setting -SK:mchr to values >4095 led to am integer overflow and subsequent poor assemblies ... or no assembly at all (I think you used a larger value for -SK:mchr) You could: - try 2.9.44x2 with -SK:mchr=4095 - try 44x3 - or wait for a couple of minutes while I am preparing 44x4 which fixes another bug (recognition of chimeras). Regards, Bastien -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html