[mira_talk] Re: not able to resume de novo RNA-seq assembly

  • From: Bastien Chevreux <bach@xxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Sun, 15 Mar 2015 19:17:11 +0100

On 13 Mar 2015, at 11:20 , Juan Pascual Anaya <jpascualanaya@xxxxxxxxx> wrote:
> I was trying to de novo assemble a transcriptome of a vertebreate, with some 
> 300M reads (including HiSeq and MiSeq), using MIRA 4.9.3.

Maybe MIRA is not the best tool for such large jobs.

> So, my problem is that due to allocation times in a cluster, MIRA is 
> obviously stopped. So when I try to resume it, it just doesn't because it 
> says it doesn't find the MAF files in the chkpt folder (which is actually 
> empty). 
> Mi first log (copied with a _1 suffix before resuming) and the log of the 
> resuming are attached, as well as my manifest file. I saw in another thread 
> that the first checkpoint is after loading and preprocessing... might it be 
> that MIRA still didn't finished that when the job was killed?

That is indeed the problem, yes.

B.



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