[mira_talk] mira and introns

Hi,

since the mail from Jose covered some problems of est assembly I also have some 
questions: My strategy is to first blast and cluster ests and then to assemble
the cluster with mira. But when I use blat to map the remaining singlets (those 
that ae not assembled) against the contigs I clearly see good introns ...

Can mira handle introns ? So for example if two parts of a read match well to a 
contig but are separated by a gap of more then 100 bps would mira handle this ?

Otherwise I would have to split the reads that matched with introns and 
reassemble (assembly with backbone?) ...

best regards,

Andreas

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Andreas Petzold
Genome Analysis
Fritz Lipmann Institute
Beutenbergstrasse 11, D-07745 Jena
voice : ++49-3641-656038
fax   : ++49-3641-656038
email : andpet@xxxxxxxxxxxxxx

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