[mira_talk] Re: mid-tags

On Wednesday 18 February 2009 Sven Klages wrote:
> The Roche software itself is writing 5' and 3' trim points into the sff
> file; the 5' trim point is usually set to base position 5 (after the key
> sequence 'TCAG'). The software can (sfftools, sfffile) split sff
> archives by their MID sequences, which means for every MID found
> there is a new sff file created with the 5' trim point shifted by
> 10 bases (or whatever length of MID is configured) to 3'.
> [...]
> just my 2p,

Most valuable 2p you have there Sven.

I don't have a MID data set myself, would you care to give a short "demo" on 
how to split the data sets with the Roche software? I'd immediately integrate 
this into the walk-through for working with 454 data.

Regards,
  Bastien

-- 
You have received this mail because you are subscribed to the mira_talk mailing 
list. For information on how to subscribe or unsubscribe, please visit 
http://www.chevreux.org/mira_mailinglists.html

Other related posts: