Not to mention it will be extremely slow ;-) Google for smth like EST genome alignment and there are quite a few solutions. EMBOSS's standard est2genome is quite good but I would first create contigs of the ESTs and then map them. It has also been built for rather inbred data... estwise from Birney is a good tool. One thing you can do is use nucmer to detect any misassemblies. a On Tue, 2009-12-22 at 16:05 +0100, Bastien Chevreux wrote: > On another note: mapping ESTs to a genome will not work well as MIRA doesn't > do splicing, I need to document that in the docs. You might want to use other > mappers for this task. > > Regards, > Bastien >
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