[mira_talk] Re: less number of reads used
- From: Bastien Chevreux <bach@xxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Fri, 18 Apr 2014 08:29:01 +0200
On 18 Apr 2014, at 8:25 , Manoharan <manoharan.k@xxxxxxxxxxxxxxx> wrote:
>> You do understand what digital normalisation does, don’t you? And why the
>> “unused” reads are not really unused?
> My understanding on DM is if we have high copy genes it takes the subset of
> the data and assemble the reads. Correct me if I am wrong.
>
> I was worried 80% of data going unused. As per mira or DM all the expressed
> genes are assembled?
Correct. Though the results with DN are worse than if you go the “split data
set and subsample” route. But the latter takes more manual work.
B.
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