[mira_talk] hybrid assembly of 454/Sanger ESTs

Hello,


I have performed an hybrid assembly of 454/Sanger ESTs.
These sequences come from a fungal genome with a high AT content.
Many contig consensus sequences are very similar (they differ only by one or two positions). 
So I don't understand why reads are not assembled into a single contig.
Is this a normal situation ? What are the Mira's mechanisms/options which allow this ? 

Thanks for your help,

Emilie Tisserant
UMR 1136 INRA/UHP-Nancy 1
F-54280 Champenoux

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