Bastien Chevreux a écrit :
yes i'm agree with you but i have some problems to find the corresponding and good command line syntax in the current version (mira3rc4d)On Donnerstag 07 Januar 2010 Laurent MANCHON wrote:okay by adding parameter -OUT:sssip=no the singlets are not saved in the assembly result. so the command line syntax is (v2.9.43):Please note that 2.9.43 is one of the last versions which had misasembly problems in some cases and that you should use a newer version.Regards, Bastien
can you help me ? on v2.9.43 is : bin/mira -project=planaire -fasta=planaire.fa -job=denovo,est,normal,454 -OUT:sssip=no -notraceinfo \ -GENERAL:kcim=yes,not=3 -SK:rt=4 \ -CO:fnicpst=yes,rodirs=10,asir=yes,mr=no \ -CL:cpat=no,pec=no,pvlc=no,qc=no,bsqc=no,mbc=no,emlc=yes,mlcr=0,smlc=0,emrc=yes,mrcr=0,smrc=0 \ -AL:bip=5,bmin=10,mrs=90:egp=yes:egpl=reject_codongaps:megpp=100,mo=60 \ -AS:mrl=30,bdq=30 -SK:mnr=yes,rt=8,mmhr=6 Laurent --