Your FASTQ file is broken: somewhere, a dot character (".") was seen in
something which should have been ACGT (or a IUPAC base).
That's at least what the output says. Unfortunately, 4.0.2 did not tell you
where exactly in the file this happened. I think I fixed that in the 4.9.x
series, please give 4.9.6 a test ride.
B.
On 06 December 2016 at 16:01 Ewelina Rubin <ewelina_rubin@xxxxxxx> wrote:
I am doing a de-novo assembly of a transcriptome. I have paired reads as
fastq files. I did use these files in an assembly using Trinity and they
worked OK. I am trying to do a similar assembly with MIRA for comparison. I
used all default parameters. The log file is attached.
I am just trying to get a suggestion what could be e problem. Where to
start troubleshooting?
Thanks
--
Ewelina Rubin, PhD
Postdoctoral Research Fellow
Graduate School of Oceanography
University of Rhode Island
215 South Ferry Road
Narragansett, RI 02882
<401.874.6105>
