Nextera XT uses 1ng gDNA or cDNA. That is a huge advantage. Adrian, can you give more details on the coverage consistency, what metrics you used to measure it? Thanks! Regards, Bing On Wed, Oct 29, 2014 at 10:41 AM, Chris Hoefler <hoeflerb@xxxxxxxxx> wrote: > How minute are we talking about? TruSeq Nano can start with ~100 ng > unsheared gDNA. > > On Tue, Oct 28, 2014 at 7:03 PM, Kevin Chen <wchen20@xxxxxxxxx> wrote: > >> Hi, Bastian & Mira community >> >> I am Bing from U of Maryland. When I read your Mira manual, I notice this >> statement below. I am interested in getting more information on that. >> Because Nextera kit has its own advantages that TruSeq does not have, for >> example, working with minute amount of material, I have high hope for >> Nextera. Do you have any prelim data to support this statement? If this is >> true, I have to re-think a lot of my experiment design using Nextera, >> that’ll change a lot of things. >> >> >> "For de-novo assemblies, do NOT (never ever at all and under no >> circumstances) use the Nextera kit, take TruSeq. The non-random >> fragmentation behaviour of Nextera leads to all sorts of problems for >> assemblers (not only MIRA) which try to use kmer frequencies as a criterion >> for repetitiveness of a given sequence." >> >> Thank you in advance, >> Bing >> >> > > > -- > Chris Hoefler, PhD > Postdoctoral Research Associate > Straight Lab > Texas A&M University > 2128 TAMU > College Station, TX 77843-2128 >