[mira_talk] TGICL and Mira

Hi,
I have made clustering with TGICL (TIGR software assembler) and MIRA on the same input of data (276 000 ESTs) and the results are very different, with TGICL i obtain 76000 contigs and 57533 singletons and for MIRA i obtain 110000 contigs and i don't know where singletons are stored. 
Why this difference ?
The both programs take 1 day of computation on server with 4 opterons processors and 64 GB memory, maybe some specific parameters can increase the speed of the computation. 


command i use with TGICL: tgicl oysterdb -l 60 -v 20 -p98
l: miminum overlap length
v: maximum length of unmatched overhangs
p: minimum percent identity for overlaps

command i use with MIRA: mira -project=oyster -fasta -estmode -AL:mrs=98


Laurent
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Laurent Manchon Email: lmanchon@xxxxxxxxxxxxxx +---------------------------------------------+

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