On 2011-10-13, at 3:46 PM, John Nash wrote: > After sending me SIX genomes in casava 1.8 format, it appears that the > SEVENTH genome came in the OLD format (as evidenced by the "/2" and "/1" at > the ends of the lines of the headers). Of course, I didn't check and just > popped the new sequence in the pipeline. My converter happily but > incorrectly converted the headers - thus removing the "/1" and "/2" at the > ends. That resulted in the error that convert_project threw when I was > trimming the CAF file to decent sized contigs. The sequence assembly looks > really weird! > > Moral: Check your fastq formatted sequences EVERY time after downloading from > the sequence provider. Upon further checking, the sequencing centre appears to have converted the file header itself because it uses the new style flow-cell IDs and Sanger offsets for quality. Hmmmmm. Check your raw data mes ami(e)s. John -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html