[mira_talk] Re: Removing a duplicate entry from a CAF file
- From: Sven Klages <sir.svencelot@xxxxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Fri, 14 Oct 2011 23:32:20 +0200
hmm, this is the "native" bcl to fastq conversion output after
demultiplexing with Illumina's CASAVA.
I am "sequencing provider"; I usually fix the header to end up with
something like /1,/2 etc.
Sven
2011/10/14 Bastien Chevreux <bach@xxxxxxxxxxxx>
> **
>
> On Friday 14 October 2011 19:29:38 Sven Klages wrote:
>
> > Is that a change in 1.8.1? In 1.8.0 though the (new) fastq header looks
>
> > like this:
>
> >
>
> > @HW-ST535:67:81C9WABXX:8:1101:2452:2200 1:N:0:ATCACG
>
> >
>
> > (note the whitespace just prior to the read number).
>
> >
>
> > 1=first read, 2=index read, 3=second read in a pe run with index. But
> this
>
> > is the same as in 1.7.
>
> > Did I get something wrong here??
>
>
> Seems like things are a bit unclear atm, maybe also depending on the
> sequencing provider.
>
>
> B.
>
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