Hello everyone, I' m using MIRA 4 in order to assemble the mitochondrial dna from sheep. I used referenced guided assembly and in the output I found (among else) one Fasta file that called unpadded which has a full sequence without any gap and a Fasta file that called padded with some gaps (" * " (star) symbol). Which is the difference between padded and unpadded files? Can I say with secure that the unpadded file is the sequence that I have assembled? Thank you very much in advance, Vasilis. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html