[mira_talk] Re: Question about illumina de-novo sequencing
- From: Bastien Chevreux <bach@xxxxxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Fri, 5 Sep 2014 09:35:54 +0200
On 05 Sep 2014, at 8:48 , Mehmet göktay <mehmetgoktay1989@xxxxxxxxx> wrote:
> About your two-fold recommendation, we expect the genome size will be about
> 900 mb. And the company will supply us 15gb and its about 10 coverage. Our
> aim is not assembling whole genome but we are planning to get long enough
> contigs to search ssr on it.
>
> Do you thing we can accomplish it with mira?
You can try: 15 gigabases is about 50m reads at 300bp. I’ve run assemblies with
more than that. You’d need quite some RAM though.
And be prepared to activate plan B for the case things go wrong or don’t work
with MIRA.
B.
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