[mira_talk] Re: Quality Values

Burkhard Steuernagel wrote:
> I will try that. Do you have experience with using BAC End Sequences? I
> was not sure if a simple hybrid assembly will work because in my case
> the Sanger Sequences cover only 2% of the BAC.
> cheers

I do the same. Because sequencing directly from BACs is not going very
well in our lab, I often add only manually curated short fasta files,
just to mark, where each BAC starts and ends. Works well.

Jan

PS: And do not forget to run ssaha with vector as first parameter. ;-)


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