[mira_talk] Re: Mappging to Reference

On Freitag 23 Juli 2010 Saulo Alves wrote:
> I'm having problems with the rail reads.
> Can you please explain to me their nature?

Ummm ... where do you see rail reads? They're not put into the results ... are 
they?

> I've noticed that a good deal of my sequences have only the rr covering it.
>  eg
> 
> ref      aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
> read1 aaaaaaaaaa
> read2                             aaaaaaaaaaaaaaaaa
> rr1                aaaaaaaaaaaaaaa
> 
> In this case, i was assuming that the region covered only by RR are
> regions without sequencing information. Turns out, more than 80% of my
> sequence is like that. Even worse, there are several SNP/GAP/INS/DEL
> in those regions, what is a bit strange.

Could you make a screenshot depicting this please? I'd like to see exactly 
what you mean.

B.

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