> On July 10, 2014 at 2:26 PM "Offord, Victoria" <vofford@xxxxxxxxx> wrote: > I have been rather impatient and when checking on the logs found this warning: > > MIRA warncode: READGROUP_UNEXPECTED_PAIRS > Title: Unexpected pairs in readgroup > [...] > I am trying to create a merged assembly from our own Illumina HiSeq 100bp data > with the 454 reads from the Schicht project (SRA reference: SRP018375). > The annotation for this project made me think this wasn't a paired run. *sigh* Looks like I'll have to react to the SRA naming scheme problem and put some additional checks into MIRA. Victoria, does the following help you out in the mean time? //www.freelists.org/post/mira_talk/Template-segment-not-recognized,1 B.