[mira_talk] Re: MIRA (V3.0.3 (production version)) does not recognize my fastaq illumina data
- From: Paul Mireji <mireji@xxxxxxxxx>
- To: mira_talk@xxxxxxxxxxxxx
- Date: Thu, 22 Sep 2011 12:00:57 -0400
Dear Bestien,
I have implemented the suggestions and the assembly seem to be working
okay... just that I seem to be getting a memory allocation problem...I
have attached the log of events..
Kind regards
Mireji PO
On Tue, Sep 20, 2011 at 5:37 PM, Bastien Chevreux <bach@xxxxxxxxxxxx> wrote:
> On Sep 20, 2011, at 23:03 , Paul Mireji wrote:
>> I am using Mira for the first time to assemble illumina trancriptome data.
>
> Hello Paul,
>
> and then you use 3.0.3? Ouch, ouch. Don't! You absolutely do want to switch
> at least to 3.4.0 or, if the transcriptome data you have is from a higher
> eukaryote (mamal, plant) - perhaps wait for an upcoming update.
>
> But do yourself a favour: ditch that old version. The 3.0.x series is not
> suited for Illumina RNASeq data. That became somewhat usable in 3.2.x and
> even more in 3.4.0. I now have plant data to work on and the next update will
> also allow a more or less usable assembly of these with 20 to 50m reads.
>
>> I have separately tried the parameters below as indicated in the
>> manual (http://mira-assembler.sourceforge.net/docs/DefinitiveGuideToMIRA.htm)
>> to get started
>>
>> mira --project=data.fastq --job=denovo,est,accurate,solexa
>>
>> and
>>
>> mira --project=data.fastq --job=denovo,est,accurate,solexa
>
> These are identical command lines. Copy paste error? :-)
>
>> and I only get the error message below.... where could I be going wrong?
>>
>> Loading data (Solexa) from FASTQ files,
>>
>> Fatal Error (may be due to problems of the input data):
>> "Could not open FASTQ file 'Test_CLC_export.fastq_in.solexa.fastq'. Is
>> it present? Is it readable? Did you want to load your data in another
>> format?"
>
> Hmmm, is there anything unclear with that error message? MIRA tried to open a
> file called "Test_CLC_export.fastq" but there wasn't any, so it stopped.
>
> Which, by the way, tells me you have not started MIRA with the lines you
> pasted above, but with something else. Because if you had started MIRA with
> what you wrote you did (mira --project=data.fastq
> --job=denovo,est,accurate,solexa), MIRA would have complained with the same
> error message but complained about a non-existent file
> "data.fastq_in.solexa.fastq". You do not need to be afraid to write the term
> "CLC" on this mailing list ... I don't bite when reading about other
> assemblers :-)
>
> Back to your problem:
>
> 1) Name your FASTQ file like this: "Test_CLC_export_in.solexa.fastq"
> 2) Start MIRA with: "mira --project=Test_CLC_export
> --job=denovo,est.accurate,solexa >&log_assembly.txt"
>
> And that's it.
>
> Hope this helped,
> Bastien
>
>
>
>
> --
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>
This is MIRA V3.4.0 (production version).
Please cite: Chevreux, B., Wetter, T. and Suhai, S. (1999), Genome Sequence
Assembly Using Trace Signals and Additional Sequence Information.
Computer Science and Biology: Proceedings of the German Conference on
Bioinformatics (GCB) 99, pp. 45-56.
To (un-)subscribe the MIRA mailing lists, see:
http://www.chevreux.org/mira_mailinglists.html
After subscribing, mail general questions to the MIRA talk mailing list:
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To report bugs or ask for features, please use the new ticketing system at:
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This ensures that requests don't get lost.
Compiled by: bach
Sun Aug 21 17:50:30 CEST 2011
On: Linux arcadia 2.6.38-11-generic #48-Ubuntu SMP Fri Jul 29 19:02:55 UTC 2011
x86_64 x86_64 x86_64 GNU/Linux
Compiled in boundtracking mode.
Compiled in bugtracking mode.
Compiled with ENABLE64 activated.
Runtime settings (sorry, for debug):
Size of size_t : 8
Size of uint32 : 4
Size of uint32_t: 4
Size of uint64 : 8
Size of uint64_t: 8
Current system: Linux mireji-desktop 2.6.32-33-generic #72-Ubuntu SMP Fri Jul
29 21:07:13 UTC 2011 x86_64 GNU/Linux
Parsing parameters: --project=Test_CLC_export --job=denovo,est,accurate,solexa
Parameters parsed without error, perfect.
-CL:pec and -CO:emeas1clpec are set, setting -CO:emea values to 1.
------------------------------------------------------------------------------
Parameter settings seen for:
Sanger data (also common parameters), Solexa data
Used parameter settings:
General (-GE):
Project name in (proin) : Test_CLC_export
Project name out (proout) : Test_CLC_export
Number of threads (not) : 2
Automatic memory management (amm) : yes
Keep percent memory free (kpmf) : 15
Max. process size (mps) : 0
EST SNP pipeline step (esps) : 0
Use template information (uti) : [san] yes
[sxa] yes
Template insert size minimum (tismin) : [san] -1
[sxa] -1
Template insert size maximum (tismax) : [san] -1
[sxa] -1
Template partner build direction (tpbd) : [san] -1
[sxa] -1
Colour reads by hash frequency (crhf) : no
Load reads options (-LR):
Load sequence data (lsd) : [san] no
[sxa] yes
File type (ft) : [san] fasta
[sxa] fastq
External quality (eq) : from SCF (scf)
Ext. qual. override (eqo) : no
Discard reads on e.q. error (droeqe): no
Solexa scores in qual file (ssiqf) : no
FASTQ qual offset (fqqo) : [san] 0
[sxa] 0
Wants quality file (wqf) : [san] yes
[sxa] yes
Read naming scheme (rns) : [san] Sanger Institute
(sanger)
[sxa] Solexa (solexa)
Merge with XML trace info (mxti) : [san] no
[sxa] no
Filecheck only (fo) : no
Assembly options (-AS):
Number of passes (nop) : 3
Skim each pass (sep) : yes
Maximum number of RMB break loops (rbl) : 1
Maximum contigs per pass (mcpp) : 0
Minimum read length (mrl) : [san] 80
[sxa] 20
Minimum reads per contig (mrpc) : [san] 2
[sxa] 4
Base default quality (bdq) : [san] 10
[sxa] 10
Enforce presence of qualities (epoq) : [san] yes
[sxa] yes
Automatic repeat detection (ard) : no
Coverage threshold (ardct) : [san] 2
[sxa] 2.5
Minimum length (ardml) : [san] 400
[sxa] 300
Grace length (ardgl) : [san] 40
[sxa] 20
Use uniform read distribution (urd) : no
Start in pass (urdsip) : 3
Cutoff multiplier (urdcm) : [san] 1.5
[sxa] 1.5
Keep long repeats separated (klrs) : no
Spoiler detection (sd) : no
Last pass only (sdlpo) : yes
Use genomic pathfinder (ugpf) : no
Use emergency search stop (uess) : yes
ESS partner depth (esspd) : 500
Use emergency blacklist (uebl) : yes
Use max. contig build time (umcbt) : yes
Build time in seconds (bts) : 3600
Strain and backbone options (-SB):
Load straindata (lsd) : no
Assign default strain (ads) : [san] no
[sxa] no
Default strain name (dsn) : [san] StrainX
[sxa] StrainX
Load backbone (lb) : no
Start backbone usage in pass (sbuip) : 3
Backbone file type (bft) : fasta
Backbone base quality (bbq) : 30
Backbone strain name (bsn) : ReferenceStrain
Force for all (bsnffa) : no
Backbone rail from strain (brfs) :
Backbone rail length (brl) : 0
Backbone rail overlap (bro) : 0
Also build new contigs (abnc) : yes
Dataprocessing options (-DP):
Use read extensions (ure) : [san] yes
[sxa] no
Read extension window length (rewl) : [san] 30
[sxa] 30
Read extension w. maxerrors (rewme) : [san] 2
[sxa] 2
First extension in pass (feip) : [san] 0
[sxa] 0
Last extension in pass (leip) : [san] 0
[sxa] 0
Clipping options (-CL):
Merge with SSAHA2/SMALT vector screen (msvs): [san] no
[sxa] no
Gap size (msvsgs) : [san] 10
[sxa] 1
Max front gap (msvsmfg) : [san] 60
[sxa] 2
Max end gap (msvsmeg) : [san] 120
[sxa] 2
Strict front clip (msvssfc) : [san] 0
[sxa] 0
Strict end clip (msvssec) : [san] 0
[sxa] 0
Possible vector leftover clip (pvlc) : [san] yes
[sxa] no
maximum len allowed (pvcmla) : [san] 18
[sxa] 18
Min qual. threshold for entire read (mqtfer): [san] 0
[sxa] 5
Number of bases (mqtfernob) : [san] 0
[sxa] 15
Quality clip (qc) : [san] no
[sxa] no
Minimum quality (qcmq) : [san] 20
[sxa] 20
Window length (qcwl) : [san] 30
[sxa] 30
Bad stretch quality clip (bsqc) : [san] yes
[sxa] no
Minimum quality (bsqcmq) : [san] 20
[sxa] 5
Window length (bsqcwl) : [san] 30
[sxa] 20
Masked bases clip (mbc) : [san] yes
[sxa] yes
Gap size (mbcgs) : [san] 20
[sxa] 5
Max front gap (mbcmfg) : [san] 40
[sxa] 12
Max end gap (mbcmeg) : [san] 60
[sxa] 12
Lower case clip (lcc) : [san] no
[sxa] no
Clip poly A/T at ends (cpat) : [san] no
[sxa] yes
Keep poly-a signal (cpkps) : [san] no
[sxa] no
Minimum signal length (cpmsl) : [san] 12
[sxa] 15
Max errors allowed (cpmea) : [san] 1
[sxa] 1
Max gap from ends (cpmgfe) : [san] 9
[sxa] 20000
Clip 3 prime polybase (c3pp) : [san] no
[sxa] yes
Minimum signal length (c3ppmsl) : [san] 12
[sxa] 12
Max errors allowed (c3ppmea) : [san] 2
[sxa] 2
Max gap from ends (c3ppmgfe) : [san] 9
[sxa] 9
Clip known adaptors right (ckar) : [san] no
[sxa] yes
Ensure minimum left clip (emlc) : [san] yes
[sxa] no
Minimum left clip req. (mlcr) : [san] 25
[sxa] 0
Set minimum left clip to (smlc) : [san] 30
[sxa] 0
Ensure minimum right clip (emrc) : [san] no
[sxa] no
Minimum right clip req. (mrcr) : [san] 10
[sxa] 10
Set minimum right clip to (smrc) : [san] 20
[sxa] 20
Apply SKIM chimera detection clip (ascdc) : yes
Apply SKIM junk detection clip (asjdc) : no
Propose end clips (pec) : yes
Bases per hash (pecbph) : 31
Handle Solexa GGCxG problem (pechsgp) : yes
Clip bad solexa ends (cbse) : yes
Parameters for SKIM algorithm (-SK):
Number of threads (not) : 2
Also compute reverse complements (acrc) : yes
Bases per hash (bph) : 17
Hash save stepping (hss) : 1
Percent required (pr) : [san] 65
[sxa] 95
Max hits per read (mhpr) : 30
Max megahub ratio (mmhr) : 0
SW check on backbones (swcob) : no
Freq. est. min normal (fenn) : 0.4
Freq. est. max normal (fexn) : 1.6
Freq. est. repeat (fer) : 1.9
Freq. est. heavy repeat (fehr) : 8
Freq. est. crazy (fecr) : 20
Mask nasty repeats (mnr) : yes
Nasty repeat ratio (nrr) : 100
Repeat level in info file (rliif) : 6
Max hashes in memory (mhim) : 15000000
MemCap: hit reduction (mchr) : 4096
Pathfinder options (-PF):
Use quick rule (uqr) : [san] yes
[sxa] yes
Quick rule min len 1 (qrml1) : [san] 200
[sxa] -95
Quick rule min sim 1 (qrms1) : [san] 90
[sxa] 100
Quick rule min len 2 (qrml2) : [san] 100
[sxa] -85
Quick rule min sim 2 (qrms2) : [san] 95
[sxa] 100
Backbone quick overlap min len (bqoml) : [san] 150
[sxa] 20
Max. start cache fill time (mscft) : 5
Align parameters for Smith-Waterman align (-AL):
Bandwidth in percent (bip) : [san] 20
[sxa] 20
Bandwidth max (bmax) : [san] 130
[sxa] 80
Bandwidth min (bmin) : [san] 25
[sxa] 20
Minimum score (ms) : [san] 30
[sxa] 15
Minimum overlap (mo) : [san] 17
[sxa] 25
Minimum relative score in % (mrs) : [san] 65
[sxa] 90
Solexa_hack_max_errors (shme) : [san] 0
[sxa] 0
Extra gap penalty (egp) : [san] no
[sxa] yes
extra gap penalty level (egpl) : [san] low
[sxa] reject_codongaps
Max. egp in percent (megpp) : [san] 100
[sxa] 100
Contig parameters (-CO):
Name prefix (np) :
Test_CLC_export
Reject on drop in relative alignment score in % (rodirs) : [san] 25
[sxa] 15
Mark repeats (mr) : yes
Only in result (mroir) : no
Assume SNP instead of repeats (asir) : no
Minimum reads per group needed for tagging (mrpg) : [san] 2
[sxa] 4
Minimum neighbour quality needed for tagging (mnq) : [san] 20
[sxa] 20
Minimum Group Quality needed for RMB Tagging (mgqrt) : [san] 30
[sxa] 30
End-read Marking Exclusion Area in bases (emea) : [san] 1
[sxa] 1
Set to 1 on clipping PEC (emeas1clpec) : yes
Also mark gap bases (amgb) : [san] yes
[sxa] yes
Also mark gap bases - even multicolumn (amgbemc) : [san] yes
[sxa] yes
Also mark gap bases - need both strands (amgbnbs): [san] yes
[sxa] yes
Force non-IUPAC consensus per sequencing type (fnicpst) : [san] no
[sxa] no
Merge short reads (msr) : [san] no
[sxa] yes
Keep ends unmerged (msrkeu) : [san] -1
[sxa] -1
Gap override ratio (gor) : [san] 66
[sxa] 66
Edit options (-ED):
Automatic contig editing (ace) : [san] no
[sxa] no
Sanger only:
Strict editing mode (sem) : no
Confirmation threshold in percent (ct) : 50
Misc (-MI):
Stop on NFS (sonfs) : yes
Extended log (el) : no
Large contig size (lcs) : 500
Large contig size for stats(lcs4s) : 1000
Stop on max read name length (somrnl) : 40
Directories (-DI):
Working directory :
When loading EXP files :
When loading SCF files :
Top directory for writing files : Test_CLC_export_assembly
For writing result files :
Test_CLC_export_assembly/Test_CLC_export_d_results
For writing result info files :
Test_CLC_export_assembly/Test_CLC_export_d_info
For writing tmp files :
Test_CLC_export_assembly/Test_CLC_export_d_tmp
Tmp redirected to (trt) :
For writing checkpoint files :
Test_CLC_export_assembly/Test_CLC_export_d_chkpt
File names (-FN):
When loading sequences from FASTA : [san]
Test_CLC_export_in.sanger.fasta
[sxa]
Test_CLC_export_in.solexa.fasta
When loading qualities from FASTA quality : [san]
Test_CLC_export_in.sanger.fasta.qual
[sxa]
Test_CLC_export_in.solexa.fasta.qual
When loading sequences from FASTQ : [san]
Test_CLC_export_in.sanger.fastq
[sxa]
Test_CLC_export_in.solexa.fastq
When loading project from CAF :
Test_CLC_export_in.sanger.caf
When loading project from MAF (disabled) :
Test_CLC_export_in.sanger.maf
When loading EXP fofn :
Test_CLC_export_in.sanger.fofn
When loading project from PHD : Test_CLC_export_in.phd.1
When loading strain data :
Test_CLC_export_straindata_in.txt
When loading XML trace info files : [san]
Test_CLC_export_traceinfo_in.sanger.xml
[sxa]
Test_CLC_export_traceinfo_in.solexa.xml
When loading SSAHA2 vector screen results :
Test_CLC_export_ssaha2vectorscreen_in.txt
When loading SMALT vector screen results :
Test_CLC_export_smaltvectorscreen_in.txt
When loading backbone from MAF :
Test_CLC_export_backbone_in.maf
When loading backbone from CAF :
Test_CLC_export_backbone_in.caf
When loading backbone from GenBank :
Test_CLC_export_backbone_in.gbf
When loading backbone from GFF3 :
Test_CLC_export_backbone_in.gff3
When loading backbone from FASTA :
Test_CLC_export_backbone_in.fasta
Output files (-OUTPUT/-OUT):
Save simple singlets in project (sssip) : [san] no
[sxa] no
Save tagged singlets in project (stsip) : [san] yes
[sxa] yes
Remove rollover tmps (rrot) : yes
Remove tmp directory (rtd) : no
Result files:
Saved as CAF (orc) : yes
Saved as MAF (orm) : yes
Saved as FASTA (orf) : yes
Saved as GAP4 (directed assembly) (org) : no
Saved as phrap ACE (ora) : yes
Saved as GFF3 (org3) : no
Saved as HTML (orh) : no
Saved as Transposed Contig Summary (ors) : yes
Saved as simple text format (ort) : no
Saved as wiggle (orw) : no
Temporary result files:
Saved as CAF (otc) : yes
Saved as MAF (otm) : no
Saved as FASTA (otf) : no
Saved as GAP4 (directed assembly) (otg) : no
Saved as phrap ACE (ota) : no
Saved as HTML (oth) : no
Saved as Transposed Contig Summary (ots) : no
Saved as simple text format (ott) : no
Extended temporary result files:
Saved as CAF (oetc) : no
Saved as FASTA (oetf) : no
Saved as GAP4 (directed assembly) (oetg) : no
Saved as phrap ACE (oeta) : no
Saved as HTML (oeth) : no
Save also singlets (oetas) : no
Alignment output customisation:
TEXT characters per line (tcpl) : 60
HTML characters per line (hcpl) : 60
TEXT end gap fill character (tegfc) :
HTML end gap fill character (hegfc) :
File / directory output names:
CAF : Test_CLC_export_out.caf
MAF : Test_CLC_export_out.maf
FASTA : Test_CLC_export_out.unpadded.fasta
FASTA quality : Test_CLC_export_out.unpadded.fasta.qual
FASTA (padded) : Test_CLC_export_out.padded.fasta
FASTA qual.(pad): Test_CLC_export_out.padded.fasta.qual
GAP4 (directory): Test_CLC_export_out.gap4da
ACE : Test_CLC_export_out.ace
HTML : Test_CLC_export_out.html
Simple text : Test_CLC_export_out.txt
TCS overview : Test_CLC_export_out.tcs
Wiggle : Test_CLC_export_out.wig
------------------------------------------------------------------------------
Deleting old directory Test_CLC_export_assembly ... done.
Creating directory Test_CLC_export_assembly ... done.
Creating directory Test_CLC_export_assembly/Test_CLC_export_d_tmp ... done.
Creating directory Test_CLC_export_assembly/Test_CLC_export_d_results ... done.
Creating directory Test_CLC_export_assembly/Test_CLC_export_d_info ... done.
Creating directory Test_CLC_export_assembly/Test_CLC_export_d_chkpt ... done.
Tmp directory is not on a NFS mount, good.
Localtime: Thu Sep 22 11:49:39 2011
Loading data (Solexa) from FASTQ files,
Localtime: Thu Sep 22 11:49:39 2011
Counting sequences in FASTQ file: found 42325367 sequences.
Localtime: Thu Sep 22 11:52:04 2011
Solexa will load 42325367 reads.
Longest Sanger: 0
Longest 454: 0
Longest IonTor: 0
Longest PacBio: 0
Longest Solexa: 74
Longest Solid: 0
Longest overall: 74
Total reads to load: 42325367
Reserving space for reads (this may take a while)
tcmalloc: large alloc 9819488256 bytes == (nil) @
========================== Memory self assessment ==============================
Running in 64 bit mode.
Dump from /proc/meminfo
--------------------------------------------------------------------------------
MemTotal: 1598568 kB
MemFree: 13828 kB
Buffers: 25952 kB
Cached: 855056 kB
SwapCached: 0 kB
Active: 619732 kB
Inactive: 844384 kB
Active(anon): 444232 kB
Inactive(anon): 149224 kB
Active(file): 175500 kB
Inactive(file): 695160 kB
Unevictable: 0 kB
Mlocked: 0 kB
SwapTotal: 3191336 kB
SwapFree: 3191336 kB
Dirty: 16 kB
Writeback: 0 kB
AnonPages: 582996 kB
Mapped: 114540 kB
Shmem: 10460 kB
Slab: 40216 kB
SReclaimable: 19708 kB
SUnreclaim: 20508 kB
KernelStack: 3296 kB
PageTables: 24464 kB
NFS_Unstable: 0 kB
Bounce: 0 kB
WritebackTmp: 0 kB
CommitLimit: 3990620 kB
Committed_AS: 1641596 kB
VmallocTotal: 34359738367 kB
VmallocUsed: 39240 kB
VmallocChunk: 34359694792 kB
HardwareCorrupted: 0 kB
HugePages_Total: 0
HugePages_Free: 0
HugePages_Rsvd: 0
HugePages_Surp: 0
Hugepagesize: 2048 kB
DirectMap4k: 12224 kB
DirectMap2M: 1622016 kB
--------------------------------------------------------------------------------
Dump from /proc/self/status
--------------------------------------------------------------------------------
Name: mira
State: R (running)
Tgid: 3792
Pid: 3792
PPid: 3567
TracerPid: 0
Uid: 1000 1000 1000 1000
Gid: 1000 1000 1000 1000
FDSize: 64
Groups: 0 1 2 3 4 5 6 7 8 9 10 12 13 15 20 21 22 24 25 26 27 29 30 33 34 37 38
39 40 41 42 43
VmPeak: 11920 kB
VmSize: 11916 kB
VmLck: 0 kB
VmHWM: 7008 kB
VmRSS: 7008 kB
VmData: 6556 kB
VmStk: 88 kB
VmExe: 5236 kB
VmLib: 0 kB
VmPTE: 40 kB
Threads: 1
SigQ: 0/16382
SigPnd: 0000000000000000
ShdPnd: 0000000000000000
SigBlk: 0000000000000000
SigIgn: 0000000000000000
SigCgt: 0000000180000000
CapInh: 0000000000000000
CapPrm: 0000000000000000
CapEff: 0000000000000000
CapBnd: ffffffffffffffff
Cpus_allowed: ff
Cpus_allowed_list: 0-7
Mems_allowed: 00000000,00000001
Mems_allowed_list: 0
voluntary_ctxt_switches: 61636
nonvoluntary_ctxt_switches: 790
--------------------------------------------------------------------------------
Information on current assembly object:
AS_readpool: 0 reads.
AS_contigs: 0 contigs.
AS_bbcontigs: 0 contigs.
Mem used for reads: 64 (64 B)
Memory used in assembly structures:
Eff. Size Free cap. LostByAlign
AS_writtenskimhitsperid: 0 24 B 0 B 0 B
AS_skim_edges: 0 24 B 0 B 0 B
AS_adsfacts: 0 24 B 0 B 0 B
AS_confirmed_edges: 0 24 B 0 B 0 B
AS_permanent_overlap_bans: 1 24 B 0 B 0 B
AS_readhitmiss: 0 24 B 0 B 0 B
AS_readhmcovered: 0 24 B 0 B 0 B
AS_count_rhm: 0 24 B 0 B 0 B
AS_clipleft: 0 24 B 0 B 0 B
AS_clipright: 0 24 B 0 B 0 B
AS_used_ids: 0 24 B 0 B 0 B
AS_multicopies: 0 24 B 0 B 0 B
AS_hasmcoverlaps: 0 24 B 0 B 0 B
AS_maxcoveragereached: 0 24 B 0 B 0 B
AS_coverageperseqtype: 0 24 B 0 B 0 B
AS_istroublemaker: 0 24 B 0 B 0 B
AS_isdebris: 0 24 B 0 B 0 B
AS_needalloverlaps: 0 40 B 0 B 0 B
AS_readsforrepeatresolve: 0 40 B 0 B 0 B
AS_allrmbsok: 0 24 B 0 B 0 B
AS_probablermbsnotok: 0 24 B 0 B 0 B
AS_weakrmbsnotok: 0 24 B 0 B 0 B
AS_readmaytakeskim: 0 40 B 0 B 0 B
AS_skimstaken: 0 40 B 0 B 0 B
AS_numskimoverlaps: 0 24 B 0 B 0 B
AS_numleftextendskims: 0 24 B 0 B 0 B
AS_rightextendskims: 0 24 B 0 B 0 B
AS_skimleftextendratio: 0 24 B 0 B 0 B
AS_skimrightextendratio: 0 24 B 0 B 0 B
AS_usedtmpfiles: 1 48 B 0 B 0 B
Total: 872 (872 B)
================================================================================
Dynamic allocs: 0
Align allocs: 0
Out of memory detected, exception message is: std::bad_alloc
If you have questions on why this happened, please send the last 1000
lines of the output log (or better: the complete file) to the author
together with a short summary of your assembly project.
For general help, you will probably get a quicker response on the
MIRA talk mailing list
than if you mailed the author directly.
To report bugs or ask for features, please use the new ticketing system at:
http://sourceforge.net/apps/trac/mira-assembler/
This ensures that requests don't get lost.
Other related posts: