You the man! keep up the good work D.
Dear all, MIRA V3 has been released at SourceForge. http://sourceforge.net/projects/mira-assembler/Documentation for MIRA can be found both in the binary packages and on the MIRA-Wiki, the latter being here:http://sourceforge.net/apps/mediawiki/mira-assembler/ So, what's new in this release? Actually, a lot!The 3.0 version of MIRA is the result of a long development to make de-novo and mapping assemblies of Sanger, 454 and Solexa (Illumina) data as easy and straightforward as possible while keeping a maximum accuracy.Another focus was to make it possible to use results from Solexa mapping projects in current finishing programs, not only viewers. MIRA introduces for that the notion of coverage equivalent reads (CER) which reduces the data volume by 70 to 90%. This allows painless use of such data sets in "gap4" and "consed".A third focus was to reduce time for researchers to find and evaluate differences in mapping assemblies. MIRA supports that by setting tags and places of interest and creating easy to understand HTML files and tables ready to be imported into spreadsheet programs.As a lot has changed since the 2.8.x series of MIRA, the following list has just a few highlights which came in during the 2.9.x series:- sequencing technologies: MIRA handles different sequencing technologies independently from each other and has specialised routine for working with each of them. - command-line parameters: MIRA has now a handful of "Do-What-I-Mean" one-stop switches which allows to configure the assembler for 90% of all use cases. Furthermore, many parameters can be adjusted for each sequencing technology so that the assembly engine can be tweaked for very specialised cases if needed. - all sequencing technologies (Sanger, 454, Solexa) have now - recognition of chimeras - new assembly routines to for improved repeat resolving - improved data preprocessing that gets rid of low quality data and sequencing errors at ends of reads ... even when no quality data is available. - 454 data: - fully developed capability for de-novo and mapping assembly of 454 data (paired and non-paired) - automated contig editor to remove most obvious and/or annoying sequencing errors - improved consensus calling streamlined to minimise the dreaded homopolymer problem - Solexa data - can handle Solexa data of any length, no restriction to very short sequences. - memory/space saving: MIRA has special mapping mode which creates data so that widely used finishing tools like gap4 and consed can load these projects and still be fairly quick - alignments enriched with features: MIRA adds information like repetitiveness or repeat marker bases as tags in the assembly so that these can be used during finishing - assembly information files: MIRA writes more information files which can be easily parsed and/or read - mapping assemblies: MIRA has a full SNP analysis for prokaryotic data - comprehensive tables and HTML result files (mapping assembly): the convert_project program can now create easy to use tables and HTML files which show the data in a way suited for less computer-interested people (biologists etc. :-) - memory management: MIRA can now be told to use an upper limit of RAM. - file formats: MIRA can now parse or write more file formats. Notable are change from SSAHA to SSAHA2 for clipping, FASTQ for data input and MAF format for output. - MacOS support: MIRA now compiles on MacOS-X - speed and memory: compared to 2.8.x, MIRA now uses way less memory and is a lot faster. - tons of other features, tweaks and bug fixes. See the CHANGES_old.txt file Have a lot of fun with MIRA :-) Bastien
-- Davide Sassera Sezione di Patologia Generale e ParassitologiaDipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria Facoltà di Veterinaria
Università degli Studi di Milano Via Celoria 10, 20133, Milano, ITALY Tel: +39 0250318094 Fax: +39 0250318095 -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html