On Sunday 13 February 2011 14:21:31 walter kiprotich wrote: > I am experiencing problems with assembling and SNPs search (ver: > mira_3.2.1_linux-gnu_x86_64) I use miraSearchESTSNPs pipeline to assemble > 454 reads, but the step3_info_snplist.txt file does not contain any SNPs > even though i can find in log file for example: ... Found > - 0 new Strong RMB (SRMc) > - 0 new Weak RMB (WRMc) > - 244 SNP > positions tagged.Transfering reads to readpool. > Done................... > Saving SNP list to file: step3_d_info/step3_info_snplist.txt What is the > problem with my miraSearchESTSNPs pipeline. All my input files are okey > and steps 1&2 are perfect with all files having results walter kosgei The snplist file is for other uses: when mapping or assembling with data which has GenBank annotation attached. Your data obviously has not. However, what you are searching for is in the step3_d_info/step3_info_consensustaglist.txt file. Search for SROc, SAOc and SIOc tags. MCVc might also apply if not at the start or end of a contig. B. -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html