I have around 130 contigs (>500 and depth >24) for bacterial genome after assembling with MIRA for SOLEXA and 454 datasets. I want to create a reference genome out of these contigs. Any ideas how can I go for scaffolding. I tried to use SSPACE but it still leaves me with around 80 contigs. Anyone used software like Projector/Genomap to close gaps? Arhn