[mira_talk] Fwd: mira3rc4 fails with small data set

• From: Bastien Chevreux <bach@xxxxxxxxxxxx>
• To: mira_talk@xxxxxxxxxxxxx
• Date: Wed, 4 Nov 2009 20:07:04 +0100

Hi Bastien, 
 
> I'm not sure this is  relevant to the mailing-list, but feel free to answer$
> me on the list if you think it is. 

Hello Jorge,

yes, in a sense it is relevant.
 
> mira ends writing this out : 
> Fatal Error (may be due to problems of the input data): 
> "No read can be used for assembly." 
> ->Thrown: void Assembly::dumpSomeStatistics() 
> ->Caught: main 
> Program aborted, probably due to error in the input data or parametrisation. 
> Please check the output log for more information 
> 
> I've checked my sequences, and they look ok. 
> I've attached my seqs along with their qualities to this e-mail, 
> could you please tell me why mira trashes them all ? (there are only 9) 
> I've tried with a simple command like : 
> mira -project=myproj -job=denovo,genome,accurate,454 -notraceinfo 
> Thank you for your help 

These sequences are being thrown out because of the proposed end clip 
(-CL:pec) which is switched on by default for genome assemblies (have a look 
at the log, it tells you it killed 9 sequences).

There's warning in the manual: 
  "Extremely effective, but should NOT be used for very low coverage data or
  for EST projects (if one wants to retain rare transcripts)."
The sequences in your example are low coverage and assemble all in forward 
direction. The routines for proposed end clips cut away everything which 
contains errors at end of reads AND which is not covered by a read in reverse 
direction. This is the case for 99...% of all reads in data from genome 
shotgun sequencing (even Sanger at 6x or 7x coverage), but not for your data 
set. Therefore, everything is cut away and no sequence remains to be 
assembled.

The MIRA "--job" defaults are made to work through some of the worst possible 
junk in the sequencing data (which happens more often than not) and are 
therefore not suited for toy data sets like these.

Solution in those cases is simple: -CL:pec=off at the end of the command line.

Regards,
  Bastien

 
>FT9JLZY01A9FK7 rank=0058788 x=400.5 y=1433.0 length=180 
AGTACGAGATCTCACAGAACGCCTACATCAAACTCGTCCTCCACTCCCTTCGCCACAAGA 
CGGCGGCGGTCAACGGAGTCCTTGTCGGTCGAATCAGCCCTAAGGACGAAGGAGTCGTGG 
AGATCTCCGATTCCGTGCCTCTCTTTCACTCCAACCTCCCCNCCTTCCTCCTCTCGAANA 
>FT9JLZY01CJDFD rank=0118285 x=923.0 y=2599.5 length=120 
TAATCGATTCCCCACGATTATCTCATTCTCTCTCGAGTCGACGATCCCAAGGTTAGAAAG 
ACGACATCTTTATTGTGTCCTTCTGAGCTTATAAGCAACTAGTTTAACCAAAAACGTTTT 
>FT9JLZY01A74NC rank=0123223 x=385.5 y=2038.0 length=561 
CAAGTGAGGCCAGGTATGTGCTTGACCTTAGAATATCCAATATCTACTTTATGTTTCAGA 
AAACTTCCCCTCTCGTGGAAGAAATGTTTTGGAGTTCTCTCCAAAATGTCAGGCATAGCC 
GCCTATATATATGCATTAACATTGAATAAAATACCGCATAGTTAAATATTTTGTTTACTC 
GTACAATAAGAGCAAGAGAGTAACCCAAGTTTAACGAAATCAGACCGTCCTTACCTGAAT 
AACAGTCGGAGGTTTGGCATTTATCTGGAGAAATTCGTTGGCAGCTTGTAATATCTGTCC 
ACAATAACAAAAGGGTTAGACCAAAAATTAAAGACAAACAATTTACAGGTTTAATTATCA 
AATCTAGTATATGGGCCGAAGCTTAGAATGATGTGTTAAAAAGGATGGGACCTTAGTGTT 
TCTGAAGACACCGTCTAGATCGTGCAGCGCGAGCCAGCCAGTTCGCCATCCTGGGACATT 
CCATCCCTTAGATAAGGATCCGAGTGTAACCACTGGTACGGTGGAAGAAAACTTTCCCAT 
CGGCACAAAAGGGTTATTCCC 
>FT9JLZY01CCXRK rank=0123352 x=850.0 y=1374.5 length=453 
CCACTTTTACTCGGAAAAACAAAGAACTACGAATGGCTTGATCTTCCGCAAAGGAAGTAC 
GTAGGCAGCCTTAACGGGTCCAGCTATAATAAAAAAAACAACACTTCCCCGAGAAGTCGC 
GCAACCCATGCGATGCCTACATTAGATTCGCCCCGGCACTTCGAGACAAACATACCGGCA 
CTCGCACCCCACAGCCGAGTATGTCCTGATCAGACACATACTCGCGGGAATCCGGTCGTA 
TCACAGTATTCACTGATACGTCCGAAATGATGACTCCTATCCATTTCATAATTTACTAAG 
TAGGGTCTTTGGCCGACCTAACGCTTAGAAATTGTTCTGAAAGTCGATGTAGGAACCGTT 
ATCCTTTGAAAATTTATCTTTCACGGTCTGAAGTTATCCTATTTTCACTCGAACAAACCA 
GGAAGCTTGATAAACGACCCAACGACACCTGAT 
>FT9JLZY01BXZU6 rank=0124371 x=680.5 y=576.0 length=454 
CCACTTTTACTCGGAAAAACAAAGAACTACGAATGGCTTGATCTTCCGCAAAGGAAGTAC 
GTAGGCAGCCTTAACGGGTCCAGCTATAATAAAAAAACAAACACTTCCCCGACGAAGTCG 
CGCAACCCATGCGATGCCTACATTAGATTCGCCCCGGCACTTCGAGACAAACATACCGGC 
ACTCGCACCCCACAGCCGAGTATGTCCTGATCAGACACATACTCGCGGGAATCCGGTCGT 
ATCACAGTATTCACTGATACGTCCGAAATGATGACTCCTATCCATTTCATAATTTACTAA 
GTAGGGTCTTTGGCCGACCTAACGCTTAGAAATTGTTCTGAAAGTCGATGTAGGAACCGT 
TATCCTTTGAAAATTTATCTTTCACGGTCTGAAGTTATCCTATTTTCACTCGAACAAACC 
AGGAAGCTTGATAAACGACCCAACGACACCTGAT 
>FT9JLZY01CAK0O rank=0148213 x=823.5 y=2134.0 length=453 
CCACTTTTACTCGGAAAAACAAAGAACTACGAATGGCTTGATCTTCCGCAAAGGAAGTAC 
GTAGGCAGCCTTAACGGGTCCAGCTATAATAAAAAAAACAACACTTCCCCGAGAAGTCGC 
GCAACCCATGCGATGCCTACATTAGATTCGCCCCGGCACTTCGAGACAAACATACCGGCA 
CTCGCACCCCACAGCCGAGTATGTCCTGATCAGACACATACTCGCGGGAATCCGGTCGTA 
TCACAGTATTCACTGATACGTCCGAAATGATGACTCCTATCCATTTCATAATTTACTAAG 
TAGGGTCTTTGGCCGACCTAACGCTTAGAAATTGTTCTGAAAGTCGATGTAGGAACCGTT 
ATCCTTTGAAAATTTATCTTTCACGGTCTGAAGTTATCCTATTTTCACTCGAACAAACCA 
GGAAGCTTGATAAACGACCCAACGACACCTGAT 
>FT9JLZY01AXDZO rank=0185372 x=263.0 y=642.5 length=360 
AGCGCCACCAATTGCATCTCTCAGCGTTTTCTGGTCGGAAAAAACCTAATCGATTCTGGA 
ATTTTAGGACGGAGATTCCTTTTTATCGTTAAACACTTCGGTAGGCCCACCCAATGTTTT 
GTCGAGGTTATCTCCATGGGCCTATCTATCGTTAATTCAATTTCATGTTCGATTTGCAAA 
AATACTCATATCTGTACAAAAAAAACNGATATTAATATGTAATATATATATATATATATA 
TAAACGAAATAAATACAAAATAATAATATTTACTAAGAATGGATTCATTATATGAGTATA 
TATACACACATATAAGAAGTTATATACAATTATATTATATGTAAATTTATAATATTCTAA 
>FT9JLZY01A5XF7 rank=0252525 x=360.0 y=1797.0 length=413 
ATCCCTTACAATAGTTCTGGTTCAGTTAATTTTTTTTCTCTCTGAGTTTTGTTATGGATT 
ATTGTTTTTTTTTTAATTGTGCAGGTTTCTTCTGTGAAAGTGATTCGTAACAAGCTAACA 
TGTCAATCAGAAGGGTATGGTTTTGTTGAGTTTCTTACACGTGCTGCAGCTGAAGAGGTT 
CTTCAGAACTTTAACGGTTCAGTGATGCCAAACTCTGAGCAGCTCTTCCGTCTAAACTGG 
GCATCTTTTAGCACTGGTGAGAAGAGAGCAGTTGAGAATAGTCCGGAGCTATCTATATTC 
GTTGGAGACTTGTCTCCGGATGTGACTGACNCTTTATTGCAAGAGCTCTTTGTTGAGAGG 
ATATCCATCTGTCAAGNGCGCTAAAGTTGTGATCGATTCCAACACTGGACGGT 
>FT9JLZY01CH0C9 rank=0292658 x=908.5 y=459.5 length=355 
GGCAGAGTTTGAGCTCCCAAACGCTCTTTACGTCTCGTAGCTAGATCTCGTAATCGATTC 
CCACGATTATCTCATTCTCTCTCGAGTCGACGATCCCAAGGTTAGAAAGACGACATCTTT 
AATTGTGTCCTTCTGAGCTTATAAGCAACTAGTTTAACCAAAACGTTTTACAGTTGTAAA 
GGCAGTGGCAACCCCAGACCCAGTCTTGGAAGTACCCTTGACTGAGGAAAATGTTGAAAG 
CGTCTTGGCGAAATCAGACCGTACCTTATGTCTGACTGGTGGGAATGTCGCATTGCATGA 
GATCGATGGCAATATTGTTCGAGTGAAACTTGCAGGGAGCGTGCGGTTCATGCCC 
>FT9JLZY01A9FK7  
37 37 37 37 37 37 37 37 37 37 37 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 
40 40 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 
37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 35 35 35 
37 37 37 35 35 35 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 
37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 26 26 25 37 37 35 32 
29 29 30 30 29 28 27 15 15 13 15 0 14 16 24 24 21 21 21 22 22 28 26 24 18 14 
13 12 0 11 
>FT9JLZY01CJDFD  
19 17 17 28 28 28 28 13 13 13 13 13 15 24 16 24 27 27 27 30 29 33 37 37 37 30 
30 31 34 37 35 35 35 30 28 28 28 31 32 35 32 32 32 30 30 26 26 28 20 20 
20 20 35 35 34 30 30 30 37 35 32 35 35 35 35 35 35 35 23 21 21 25 23 23 25 24 
33 33 33 33 35 35 35 30 30 30 30 32 35 35 32 21 20 20 28 30 37 37 35 33 
31 30 21 21 21 24 27 13 13 11 11 11 11 11 11 13 14 16 14 14 
>FT9JLZY01A74NC  
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 38 
38 38 38 38 38 38 38 40 40 40 40 40 40 40 40 40 40 40 38 38 38 39 39 39 
40 40 40 39 39 39 40 40 40 30 28 29 31 40 26 26 21 20 20 20 32 23 40 40 40 40 
28 28 30 30 40 34 34 34 38 38 29 29 31 31 40 40 40 40 40 40 40 40 40 40 
40 40 26 26 26 26 40 40 40 40 40 40 38 34 34 36 40 40 40 40 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 34 19 21 21 
21 34 25 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 31 31 31 31 40 37 37 37 
40 40 40 40 40 40 40 40 40 38 38 38 38 38 40 40 34 34 34 38 38 40 40 40 
40 40 40 39 39 39 40 40 40 39 39 39 40 40 40 40 38 38 38 40 40 40 40 40 40 40 
40 40 40 40 34 34 30 30 30 40 40 40 40 38 37 37 40 40 40 40 40 40 40 40 
40 40 37 37 37 40 40 40 40 20 20 20 25 34 40 34 33 33 36 36 31 31 34 36 36 34 
30 27 27 34 34 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 
40 37 30 30 30 29 40 40 29 29 29 29 34 34 34 36 36 24 34 40 40 40 22 22 22 22 
22 40 40 16 16 16 40 36 40 39 39 36 40 34 40 39 38 39 36 40 30 30 30 36 
34 34 34 34 28 28 40 40 40 34 34 34 40 38 36 29 29 30 36 36 40 40 24 24 24 30 
30 39 40 40 40 40 40 40 40 40 40 40 40 36 33 33 28 25 17 12 12 11 11 11 
11 11 12 12 15 26 12 12 12 27 27 27 22 22 22 27 24 29 32 24 24 25 21 30 30 30 
29 26 20 19 19 19 20 29 28 29 32 32 30 32 32 32 32 32 32 32 33 33 32 32 
32 32 32 32 32 32 32 32 32 32 25 25 25 25 30 28 28 29 25 20 20 19 29 31 31 32 
30 27 25 25 25 25 32 30 15 15 15 19 19 25 30 29 20 19 19 32 32 30 29 29 
29 32 32 31 32 32 32 32 32 32 29 22 22 25 25 25 30 32 32 17 17 19 17 17 25 25 
26 21 21 21 21 25 25 25 29 27 27 24 27 27 22 15 15 15 18 15 11 11 11 11 
11 11 11 15 15 12 11 11 11 11 11 
>FT9JLZY01CCXRK  
40 40 40 36 20 20 20 19 24 26 30 40 36 36 20 22 23 23 23 40 38 38 38 40 40 40 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 
39 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 39 39 39 39 39 
39 39 40 40 40 40 40 40 40 40 40 40 40 40 16 16 16 16 16 16 16 16 40 21 
21 40 40 40 40 40 34 34 34 33 39 39 39 40 40 40 40 40 40 40 40 40 40 40 39 39 
39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 35 
26 26 26 26 39 39 30 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 34 34 34 34 40 40 40 40 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 
39 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 40 40 40 40 
40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 39 39 39 40 40 28 34 34 40 
40 36 20 20 20 32 33 33 34 34 26 26 26 26 35 39 40 40 40 40 40 40 40 40 40 40 
39 30 30 30 30 40 40 40 40 40 40 36 34 26 26 26 40 40 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 36 36 35 40 31 31 31 29 22 24 24 16 
40 40 34 34 34 36 40 40 40 40 40 40 40 35 34 34 40 40 40 40 40 40 40 40 
40 32 32 32 32 40 39 39 39 39 40 40 40 39 34 34 38 40 39 28 31 31 31 31 35 36 
36 36 36 36 36 35 35 35 36 36 40 35 35 35 36 36 40 36 40 36 36 36 36 36 
36 36 36 
>FT9JLZY01BXZU6  
40 40 40 40 31 31 31 31 40 40 30 40 27 27 14 14 14 14 14 32 15 15 15 34 27 30 
36 36 36 38 39 39 39 40 40 40 40 40 40 40 40 40 40 34 34 34 34 40 40 38 
38 38 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 
39 39 40 40 40 40 40 40 40 40 40 36 34 23 12 12 12 12 12 11 11 22 10 10 
10 12 15 28 25 28 19 19 22 22 24 26 17 26 30 40 40 40 40 40 40 40 40 40 40 39 
39 39 39 39 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 36 30 
30 18 18 18 18 24 24 23 31 29 32 32 36 40 40 40 40 40 30 30 30 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 35 26 26 26 26 30 19 39 39 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 
39 39 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 30 30 30 35 39 40 40 40 34 34 34 40 40 40 40 40 
40 40 40 36 36 36 40 40 26 30 30 34 34 30 30 30 40 40 40 40 40 40 40 40 40 40 
40 35 30 28 28 24 39 39 40 40 40 40 40 39 39 38 38 40 40 40 40 40 40 35 
35 35 40 40 34 34 34 34 40 40 40 40 40 40 40 39 39 39 40 32 32 34 34 39 39 39 
40 40 40 39 39 39 40 40 40 40 40 40 40 39 39 39 39 39 40 40 40 40 40 40 
40 40 33 33 33 33 40 40 40 40 37 33 33 33 32 28 22 22 24 22 19 32 32 34 34 31 
34 36 36 36 36 34 32 32 32 31 31 24 32 33 33 36 36 31 36 35 36 31 31 31 
34 36 36 36 
>FT9JLZY01CAK0O  
40 40 40 40 34 34 34 31 40 40 33 40 30 30 18 18 18 18 18 27 26 30 30 40 37 37 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 
39 39 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 39 
39 39 40 40 40 40 40 40 40 40 33 33 28 19 12 12 12 12 12 12 12 11 22 16 
16 30 24 33 32 36 27 29 29 29 30 30 30 34 40 40 40 40 40 40 40 40 40 40 39 39 
39 39 39 39 40 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 39 
31 31 31 31 39 39 30 40 40 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 34 34 34 33 40 40 40 40 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 34 
34 34 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 
40 40 40 40 40 40 40 40 40 40 40 40 40 40 30 30 30 40 40 40 40 40 40 40 40 36 
39 39 40 40 40 40 35 27 27 27 30 39 40 40 40 34 34 34 40 40 28 34 34 35 
40 39 38 37 37 40 40 36 39 39 39 39 39 39 40 40 40 40 40 30 30 30 39 40 40 39 
39 39 38 39 39 40 40 40 40 40 40 40 40 39 39 39 40 40 40 40 40 35 30 30 
30 40 40 40 40 40 40 40 40 40 40 36 36 32 22 23 23 25 15 15 15 15 15 15 15 16 
22 32 22 22 24 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 
40 34 34 34 34 40 35 35 35 39 40 40 40 40 38 28 28 30 30 24 36 36 36 36 33 34 
34 27 27 27 34 33 33 33 34 31 24 22 22 22 32 32 31 36 33 36 36 36 36 36 
36 36 36 
>FT9JLZY01AXDZO  
35 35 32 31 31 35 37 37 37 37 37 37 37 37 39 39 39 37 39 37 37 37 37 37 37 37 
23 23 23 23 37 37 37 37 37 37 37 37 21 22 24 24 22 22 40 40 40 40 40 40 
40 39 37 35 33 33 33 33 35 30 30 21 21 21 18 24 33 33 33 32 32 32 30 32 33 33 
33 28 28 20 17 17 17 17 27 20 30 30 27 27 19 20 21 30 32 37 37 37 37 37 
37 37 37 37 37 37 37 37 35 27 27 27 33 33 32 21 21 18 18 18 25 22 35 35 35 35 
35 35 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 
37 37 37 37 37 37 37 37 37 37 35 35 35 37 37 37 37 37 37 37 37 37 21 21 21 32 
37 29 29 29 29 26 32 18 32 37 37 37 37 37 35 33 33 27 23 24 24 14 14 14 
14 14 14 14 14 30 0 28 28 35 35 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 
37 37 37 37 37 37 37 37 37 37 37 35 25 25 25 28 28 28 25 20 16 16 16 27 
23 23 23 30 25 30 21 24 24 28 37 24 24 35 35 35 35 35 30 20 20 19 24 19 32 31 
31 23 19 19 19 19 24 25 23 24 30 32 32 33 33 35 35 32 32 34 37 35 35 35 
35 35 35 35 33 32 27 27 27 31 32 27 19 15 15 14 18 18 30 29 26 26 23 27 27 23 
19 19 19 19 19 23 26 27 20 20 20 22 24 22 12 13 13 13 11 11 11 11 13 18 
24 24 24 20 17 17 18 17 17 17 
>FT9JLZY01A5XF7  
37 37 37 37 37 37 37 37 37 37 37 40 40 39 39 39 39 39 37 37 37 37 37 39 39 35 
35 35 35 19 19 19 19 19 19 14 14 37 21 39 39 39 39 37 37 39 31 31 31 30 
37 37 37 37 37 30 27 27 26 24 28 23 22 19 11 11 11 11 11 11 11 11 11 11 12 12 
10 10 22 20 28 28 28 30 32 28 30 30 30 24 24 28 31 37 37 37 37 37 30 37 
37 37 37 37 37 37 35 31 31 31 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 
37 37 37 37 37 37 37 37 37 37 37 37 35 37 28 28 28 28 30 23 23 35 35 35 
37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 
37 37 37 35 35 35 35 37 34 30 31 31 37 37 37 37 37 37 37 37 37 37 37 37 
37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 37 32 
32 32 32 32 32 33 32 20 20 20 19 31 25 25 25 35 35 35 35 26 28 28 28 37 
37 37 37 35 35 35 35 34 34 25 25 25 25 35 35 35 35 35 35 35 37 35 35 35 35 35 
35 38 38 38 38 38 38 38 38 35 37 27 25 25 30 33 33 32 30 27 23 23 23 29 
24 23 23 23 23 24 23 23 30 32 32 33 33 35 35 35 35 35 35 35 35 35 35 35 35 35 
35 35 35 30 0 25 25 33 33 30 23 23 35 35 35 35 35 35 32 32 32 32 28 27 
26 24 15 15 19 24 23 17 17 17 17 25 17 17 19 23 29 25 25 26 29 31 31 31 19 18 
0 18 18 27 27 31 26 23 23 25 26 26 27 29 27 27 23 23 23 23 25 27 27 27 
23 19 17 17 17 23 23 21 22 22 20 20 22 
>FT9JLZY01CH0C9  
28 28 30 30 30 32 31 19 15 13 14 19 24 25 25 11 11 11 11 11 11 12 14 28 25 24 
21 21 21 22 19 19 18 20 25 33 33 33 32 33 33 33 33 33 35 31 28 28 32 28 
23 23 23 23 24 30 32 29 29 18 16 18 32 32 35 35 32 32 32 35 35 35 35 35 35 35 
32 30 30 30 30 34 34 37 37 37 37 37 37 37 37 37 37 37 37 37 37 35 35 35 
35 37 37 35 31 31 30 37 37 37 35 35 35 28 25 25 25 25 26 26 23 23 21 21 27 29 
19 19 19 19 32 33 32 24 25 25 33 32 32 32 35 25 25 23 27 27 32 32 33 33 
23 15 15 15 15 26 26 27 24 11 11 11 11 11 19 13 13 13 11 12 20 24 27 26 26 27 
26 23 23 23 23 23 30 30 27 24 23 23 27 23 23 19 19 19 19 18 24 27 19 19 
19 30 32 32 32 32 32 30 30 25 26 24 30 35 28 28 28 32 29 24 24 24 24 24 24 19 
19 15 15 15 15 27 31 32 31 33 28 28 28 35 35 35 35 33 33 33 20 20 20 19 
19 19 23 30 31 31 30 30 19 19 19 20 20 35 33 33 33 32 29 30 30 33 35 20 20 20 
20 25 28 28 28 28 28 31 31 33 33 30 30 30 30 35 30 30 30 35 35 35 35 35 
32 30 30 30 35 35 35 35 35 35 35 35 35 35 35 35 33 28 28 27 27 30 30 31 19 14 
14 14 24 23 28 29 30 27 19 19 19 33 32 33 33 33 33 35 32 32 32 29 28 29 
29 30 24 19 19 

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