Hello,
I am using MIRA (4.9.6) to assemble a plant genome (260MB) with illumina
and corrected PacBio data. However my assembly has aborted after the
following error:
Internal logic/programming/debugging error (*sigh* this should not have
happened)
------------------------------
- Alignment of two sequences: 81726 > 65530 bases, cannot handle,
shouldn't *
- have happened! *
------------------------------
->Thrown: void AlignedDualSeq::acquireSequences(const char * seq1, const
char * seq2, readid_t id1, readid_t id2, int8 id1dir, int8 id2dir, bool
enforce_clean_ends, bool dontpenalisengaps, int32 bandwidthused, int32
minbanddistance)
->Caught: void Align::fullAlign(std::list<AlignedDualSeq> * adslist, bool
enforce_clean_ends, bool dontpenalisengaps)
I have put the full log here :
https://www.dropbox.com/s/47stfpkn14vgqm5/Assembly_MIRA_June13r.txt.zip?dl=0
I have re-started the assembly only to get a similar error (Alignment of
two sequences: 66164 > 65530 bases, cannot handle, shouldn't have
happened!)
Is there any way to continue the assembly?
Thanks,
Beatriz
