Hi Bastien, I´ve tried with Sk:nrr=50 and I´ve increased up to 60 but I get the same message: "You have 2.61519% of your reads as megahubs. You have set a maximum allowed ratio of: 0. Ending the assembly because the maximum ration has been reached/surpassed" Do you think I should try increasing the mmhr? Maybe SK:mmhr=3?? Thanks a lot Juan El 13/10/2011, a las 12:53, Bastien Chevreux escribió: > From: Juan Fernandez Tajes<jfernandezt@xxxxxx> >> Just in case, I attach the complete repeat ratio histogram.... > > Which is way more informative than just the first couple of lines posted > previously. > > OK, EST project. Let me guess: you directly went in with nrr=10, right? > > Nothing really extraordinary from the hash statistics, but 10 is definitively > too low. Try an assembly with > > -SK:nrr=50 > > and I suppose it would run through. Maybe adjust mmhr, but maybe not > necessary. > > B. > > > -- > You have received this mail because you are subscribed to the mira_talk > mailing list. For information on how to subscribe or unsubscribe, please > visit http://www.chevreux.org/mira_mailinglists.html --------------------------------------------------------------- Juan Fernandez Tajes, ph. D Grupo XENOMAR Departamento de Biología Celular y Molecular Facultad de Ciencias-Universidade da Coruña Tlf. +34 981 167000 ext 2030 e-mail: jfernandezt@xxxxxx --------------------------------------------------------------- -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html