Hi all, I have launched mira with -job=denovo,est,accurate,454 -highlyrepetitive -notraceinfo, nevertheless, mira stops and warns me about megahubs in my read (approximately a 2%). The program and the manual suggest set -SK:nrr set to 10, Do you think that with these parameters will fix the problem: -job=denovo,est,accurate,454 -highlyrepetitive -notraceinfo -SK:mnr=yes:nrr=10 Many thanks for the help Juan El jue, 06-10-2011 a las 00:04 +0200, Bastien Chevreux escribió: > On Monday 03 October 2011 23:47:58 Juan Fernandez Tajes wrote: > > > [...] > > > -job=denovo,est,accurate,454 -highlyrepetitive -notraceinfo, are > these > > > parameters appropriate?? > > > If you're not looking for something special, yes. > > > > It would be a better idea running a "draft", or "normal" assembly > before an > > > "accurate" one? > > > Not really. > > > B. > -- You have received this mail because you are subscribed to the mira_talk mailing list. For information on how to subscribe or unsubscribe, please visit http://www.chevreux.org/mira_mailinglists.html