Hi Bobby,
You don't need to merge anything, you should compare the two results
(1st vs. 2nd assembly).
If you don't see the "double" genome in the 2nd assembly, it means the
1st assembly went wrong because of too high coverage and you can discard it.
N50 is not an indicator of accuracy, but yeah, it might even get higher
with less coverage (the warning message from MIRA explains it).
Cheers,
Andrej
On 22-Sep-16 09:10, Bobby Paul wrote:
Dear Andrej,
Thanks for your note. Obviously we will get two assemblies.
1. How to merge these two assemblies?
2. Whether it will reduce N50?
Thanks and Regards
Bobby Paul
