[mira_talk] Assembly genome pine

Hi All,
I'm using the software Mira2 for assembler my sequences (novo 454 genome) and I'm very satisfied with the result. Congratulations for the work.
Nevertheless, I'm working with sequences of pine, it is a highly repetitive DNA and I believe that they can produce mistakes of assembly. I use:
"mira -fasta -project=40B10 -job=denovo,genome,draft,454 -highlyrepetitive -LR:mxti=yes -FN:xtii=40B10_traceinfo_in.454.xml -AS:nop=10 -AS:rbl=5 -AS:mrl=3" 

What other parameters can I change to improve the result?

Hope it helps,

Rocío Bautista




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Rocío Bautista Moreno
Centro de Supercomputación y Bioinformática
Universidad de Málaga

EMAIL: rociobm@xxxxxxxxxxx          Edificio de Bioinnovación
TELEF: +34952137649               C/ Severo Ochoa 34
FAX  : +34952132790                 Parque Tecnológico de Andalucía
                                   29590 Málaga (SPAIN)




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