Thanks a lot Lionel. I was completely ignorant that in fasta also we use quality values this way. Thanks again, Rgrds, Sharmistha On Fri, Oct 2, 2009 at 7:01 PM, Lionel Guy <guy.lionel@xxxxxxxxx> wrote: > http://www.bioperl.org/wiki/Qual_sequence_format > > > On 2 Oct 2009, at 15:27 , Sharmista Saha wrote: > > Okays. >> >> Now I am following Lionel only. >> >> One quick simple doubt I know, fasta and fastq file, but what is this >> fasta qual? I did not get that? if you can let me know, it will be good. >> >> >> Thanks and regards, >> Sharmistha >> >> >> >> On Fri, Oct 2, 2009 at 6:30 PM, Bastien Chevreux <bach@xxxxxxxxxxxx> >> wrote: >> >> You are confusing the formats: >> >> FASTA + FASTA quality (http://en.wikipedia.org/wiki/FASTA_format) >> >> versus >> >> FASTQ (http://en.wikipedia.org/wiki/FASTQ_format ) >> >> As the story unravels and the puzzle comes together from the bits and >> pieces of your mails, it seems that you tried all the time to load a FASTQ >> file as FASTA quality file. This obviously cannot work. >> >> Now, please follow step by step the advice Lionel just gave you in his >> last mail and things should be running smoothly. >> >> Regards, >> Bastien >> >> >> -- >> You have received this mail because you are subscribed to the mira_talk >> mailing list. For information on how to subscribe or unsubscribe, please >> visit http://www.chevreux.org/mira_mailinglists.html >> >> > ============================================ > Lionel Guy > Thunmansgatan 25, SE-75421 Uppsala > > phone: +46 (0)18 245596 > mobile: +46 (0)73 9760618 > email: guy.lionel@xxxxxxxxx > ============================================ > > > -- > You have received this mail because you are subscribed to the mira_talk > mailing list. For information on how to subscribe or unsubscribe, please > visit http://www.chevreux.org/mira_mailinglists.html >