[mira_talk] Re: Assembly

  • From: Sharmista Saha <sharmistasaha@xxxxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Fri, 2 Oct 2009 19:15:25 +0530

Thanks a lot Lionel.
 I was completely ignorant that in fasta also we use quality values this
way.

Thanks again,
Rgrds,
Sharmistha
On Fri, Oct 2, 2009 at 7:01 PM, Lionel Guy <guy.lionel@xxxxxxxxx> wrote:

> http://www.bioperl.org/wiki/Qual_sequence_format
>
>
> On 2 Oct 2009, at 15:27 , Sharmista Saha wrote:
>
>  Okays.
>>
>> Now I am following Lionel only.
>>
>> One quick simple doubt I know, fasta and fastq file, but what is this
>> fasta qual? I did not get that? if you can let me know, it will be good.
>>
>>
>> Thanks and regards,
>> Sharmistha
>>
>>
>>
>> On Fri, Oct 2, 2009 at 6:30 PM, Bastien Chevreux <bach@xxxxxxxxxxxx>
>> wrote:
>>
>> You are confusing the formats:
>>
>> FASTA + FASTA quality   (http://en.wikipedia.org/wiki/FASTA_format)
>>
>>  versus
>>
>> FASTQ   (http://en.wikipedia.org/wiki/FASTQ_format )
>>
>> As the story unravels and the puzzle comes together from the bits and
>> pieces of your mails, it seems that you tried all the time to load a FASTQ
>> file as FASTA quality file. This obviously cannot work.
>>
>> Now, please follow step by step the advice Lionel just gave you in his
>> last mail and things should be running smoothly.
>>
>> Regards,
>>  Bastien
>>
>>
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