Hi Martin, 2009/9/9 Martin A. Hansen <mail@xxxxxxxxx> > I ran all the 454 reads (n=478840, ~270bp) and Solexa reads (n=3308974, > 35bp and 44bp pairs) along with the quality scores: > > M1_in.454.fasta > M1_in.454.qual > M1_in.solexa.fasta > M1_in.solexa.qual > M1_traceinfo_in.454.xml > > [...] No idea if this is relevant, but MIRA docs say default file naming should be something like M1_in.454.fasta M1_in.454.fasta.qual M1_in.solexa.fasta M1_in.solexa.fasta.qual Maybe your quals have not been read? cheers, Sven