[mira_talk] Re: 90% Roche454 reads going to debris file...help please !!

  • From: Bastien Chevreux <bach@xxxxxxxxxxxx>
  • To: mira_talk@xxxxxxxxxxxxx
  • Date: Sat, 8 Oct 2011 11:30:38 +0200

On Friday 07 October 2011 00:01:56 jyotsna guleria wrote:
> I tried what you suggested still it didn't work.. :(
> 
> Here is the new logfile with changes:
> 
> http://dl.dropbox.com/u/10010521/logfile


Interesting. MIRA actually assembles almost all your reads in previous passes 
into a single contig with ~6k reads and a length of ~450bp.

That is not really behaving like a normal genome, you know :-)

A routine kicks in which is there to try and find reads which spoil an 
assembly ... and it marks a lot of these reads as spoil sports.

Try this: -AS:sd=no


Bastien

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