Hi! I think there will be plenty of answers to this question.. just have a look to -LR and -AS on the MIRA manual. Perhaps something like -AS:epoq=no or -LR:mxti=no could be useful... but actually I don't remember the manual, it's just what I did. 2011/12/12 Charles Determan Jr <deter088@xxxxxxx> > Greetings, > > I am new to MIRA and have been trying to work through the walkthrough for > the 454 data. I am able to use sff_extract and rename everything according > to the document (except changing fasta to fastq format). However, when I > run the assembly with the command sudo /.../mira --project=bceti > --job=denovo,genome,accurate,454 >&log_assembly, there are no results and > the log file tells me there is no quality data. Any suggestions are > thoughts for troubleshooting this would be truly appreciated. If you > require further information or coding I have done, I can post that as well. > > Regards > -- Jordi